Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes

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Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes.

Laccases are useful biocatalysts for many diverse biotechnological applications. In this study we have established efficient and reliable expression systems and high-throughput screenings for the recombinant CotA-laccase from Bacillus subtilis. The expression levels of cotA-laccase were compared in five different Escherichia coli host strains growing in 96-well microtiter plates under different...

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ژورنال

عنوان ژورنال: Biotechnology Journal

سال: 2009

ISSN: 1860-6768,1860-7314

DOI: 10.1002/biot.200800248